Nanotheranostics 2020; 4(4):201-209. doi:10.7150/ntno.41639
A Gold Nanoparticle Bouquet held on plasma membrane: An ultrasensitive dark-field imaging approach for Cancer Cell Analysis
Department of Forensic Medicine, Nanjing Medical University, Nanjing, Jiangsu, 211166, PR China.
Cao Y, Wang J, Jiang QY, Hu L, Yu YJ, Yu YF, Chen F. A Gold Nanoparticle Bouquet held on plasma membrane: An ultrasensitive dark-field imaging approach for Cancer Cell Analysis. Nanotheranostics 2020; 4(4):201-209. doi:10.7150/ntno.41639. Available from http://www.ntno.org/v04p0201.htm
Rational: p53 is suppressing tumor protein correlated with the cell cycle factors and apoptosis. Here, a gold nanoparticle bouquet is designed for an ultrasensitive dark-field imaging approach for cancer cell analysis.
Methods: AuNP60/APBA is functionalized by a gold nanoparticle bouquet-plasmonic 60 nm gold nanoparticles. And consistent APBA can be held on the plasma membrane. After 13 nm gold nanoparticles are functionalized with mannose (AuNP13/MN), the AuNP60/APBA gold nanoparticles are captured. The absorption spectrum of aggregation gold nanoparticles (AuNPs) shifts to near-infrared (NIR) region which can be observed under dark-field microscopy (DFM) and is treated the subsequent with photothermal therapy.
Results: The results that MCF-7 cells were successfully destroyed under the near-infrared (NIR) irradiation and the intracellular WTp53 increased while the MTp53 decreased. These results indicated that p53 is the key molecule in the apoptosis signaling pathway. Photothermal therapy can stimulate the MTp53 in the cell signal conductive pathway.
Conclusion: This work offers a new method for intracellular p53 analysis and a potential targeted cancer treatment.
Keywords: gold nanoparticle bouquet, dark-field imaging, p53, cancer cell, photothermal therapy