Biogenic Zinc Oxide Nanoparticles synthesized from <i>Tinospora Cordifolia</i> induce oxidative stress, mitochondrial damage and apoptosis in Colorectal Cancer

Berehu, Hadgu Mendefro; Patnaik, Srinivas

doi:10.7150/ntno.84995

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Theranostics

International Journal of Biological Sciences

International Journal of Medical Sciences

Journal of Cancer

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Nanotheranostics 2024; 8(3):312-329. doi:10.7150/ntno.84995 This issue Cite

Research Paper

Biogenic Zinc Oxide Nanoparticles synthesized from Tinospora Cordifolia induce oxidative stress, mitochondrial damage and apoptosis in Colorectal Cancer

Hadgu Mendefro Berehu, Srinivas Patnaik^✉

Disease Biology Laboratory, School of Biotechnology KIIT Deemed to Be University, Odisha, India.

Citation:

Berehu HM, Patnaik S. Biogenic Zinc Oxide Nanoparticles synthesized from Tinospora Cordifolia induce oxidative stress, mitochondrial damage and apoptosis in Colorectal Cancer. Nanotheranostics 2024; 8(3):312-329. doi:10.7150/ntno.84995. https://www.ntno.org/v08p0312.htm

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Abstract

Cancer chemotherapy remains a serious challenge, and new approaches to therapy are urgently needed to build novel treatment regimens. The methanol extract of the stem of Tinospora Cordifolia was used to synthesize biogenic zinc oxide nanoparticles (ZnO-NPs) that display anticancer activities against colorectal cancer. Biogenic ZnO-NPs synthesized from methanol extract of Tinospora Cordifolia stem (ZnO-NPs TM) were tested against HCT-116 cell lines to assess anticancer activity. UV-Vis, FTIR, XRD, SEM, and TEM analysis characterized the biogenic ZnO-NPs. To see how well biogenic ZnO-NPs fight cancer, cytotoxicity, AO/EtBr staining, Annexin V/PI staining, mitochondrial membrane potential (MMP), generation of reactive oxygen species (ROS) analysis, and caspase cascade activity analysis were performed to assess the anticancer efficacy of biogenic ZnO-NPs. The IC50 values of biogenic ZnO-NPs treated cells (HCT-116 and Caco-2) were 31.419 ± 0.682μg/ml and 36.675 ± 0.916μg/ml, respectively. qRT-PCR analysis showed that cells treated with biogenic ZnO-NPs Bax and P53 mRNA levels increased significantly (p ≤ 0.001). It showed to have impaired MMP and increased ROS generation. In a corollary, our in vivo study showed that biogenic ZnO-NPs have an anti-tumour effect. Biogenic ZnO-NPs TM showed both in vitro and in vivo anticancer effects that could be employed as anticancer drugs.

Keywords: Anticancer, Apoptosis, biogenic ZnO-NPs, Caspase cascade, Colorectal cancer, Cytotoxicity, Green synthesis, in vivo, MMP, ROS

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APA

Berehu, H.M., Patnaik, S. (2024). Biogenic Zinc Oxide Nanoparticles synthesized from Tinospora Cordifolia induce oxidative stress, mitochondrial damage and apoptosis in Colorectal Cancer. Nanotheranostics, 8(3), 312-329. https://doi.org/10.7150/ntno.84995.

ACS

Berehu, H.M.; Patnaik, S. Biogenic Zinc Oxide Nanoparticles synthesized from Tinospora Cordifolia induce oxidative stress, mitochondrial damage and apoptosis in Colorectal Cancer. Nanotheranostics 2024, 8 (3), 312-329. DOI: 10.7150/ntno.84995.

NLM

CSE

Berehu HM, Patnaik S. 2024. Biogenic Zinc Oxide Nanoparticles synthesized from Tinospora Cordifolia induce oxidative stress, mitochondrial damage and apoptosis in Colorectal Cancer. Nanotheranostics. 8(3):312-329.

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