High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT

Tavakoli, Clément; Cuccione, Elisa; Dumot, Chloé; Balegamire, Joëlle; Si-Mohamed, Salim Aymeric; Kim, Johoon; Crola-da-Silva, Claire; Chevalier, Yves; Boussel, Loïc; Douek, Philippe; Cormode, David; Elleaume, Hélène; Brun, Emmanuel; Wiart, Marlène

doi:10.7150/ntno.79575

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Theranostics

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Nanotheranostics 2023; 7(2):176-186. doi:10.7150/ntno.79575 This issue Cite

Research Paper

High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT

Clément Tavakoli, MSc^1,2, Elisa Cuccione, PhD¹, Chloé Dumot, MD, PhD^1,3, Joëlle Balegamire, PhD⁴, Salim Aymeric Si-Mohamed, MD, PhD^5,6, Johoon Kim, PhD⁷, Claire Crola-da-Silva, PhD¹, Yves Chevalier, PhD⁴, Loïc Boussel, MD, PhD^5,6, Philippe Douek, MD, PhD^5,6, David Cormode, PhD⁷, Hélène Elleaume, PhD^2✉*, Emmanuel Brun, PhD^2*, Marlène Wiart, PhD^1,8*✉

1. Univ Lyon, CarMeN Laboratory, INSERM, INRA, INSA Lyon, Université Claude Bernard Lyon 1, 69003, Lyon, France.
2. Univ. Grenoble Alpes, Inserm UA7 Strobe, Grenoble, France.
3. Hospices Civils de Lyon, Lyon, France.
4. LAGEPP, University of Lyon 1, CNRS UMR 5007, 43 bd 11 Novembre, 69622 Villeurbanne, France.
5. Department of cardiovascular and thoracic radiology, Louis Pradel Hospital, Hospices Civils de Lyon, 59 Boulevard Pinel, 69500 Bron, France.
6. CREATIS, UMR 5220, Univ Lyon, INSA Lyon, University Claude Bernard Lyon 1, Lyon, France.
7. Department of Radiology, University of Pennsylvania, Pennsylvania, United States.
8. CNRS, Lyon, France.
*Co-last authors.

Citation:

Tavakoli C, Cuccione E, Dumot C, Balegamire J, Si-Mohamed SA, Kim J, Crola-da-Silva C, Chevalier Y, Boussel L, Douek P, Cormode D, Elleaume H, Brun E, Wiart M. High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT. Nanotheranostics 2023; 7(2):176-186. doi:10.7150/ntno.79575. https://www.ntno.org/v07p0176.htm

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Abstract

Background: The objective of this study was to demonstrate that synchrotron K-edge subtraction tomography (SKES-CT) can simultaneously track therapeutic cells and their encapsulating carrier, in vivo, in a rat model of focal brain injury using a dual-contrast agent approach. The second objective was to determine if SKES-CT could be used as a reference method for spectral photon counting tomography (SPCCT).

Methods: Phantoms containing different concentrations of gold and iodine nanoparticles (AuNPS/INPs) were imaged with SKES-CT and SPCCT to assess their performances. A pre-clinical study was performed in rats with focal cerebral injury which intracerebrally received AuNPs-labelled therapeutic cells encapsulated in a INPs-labelled scaffold. Animals were imaged in vivo with SKES-CT and back-to-back with SPCCT.

Results: SKES-CT revealed to be reliable for quantification of gold and iodine, whether alone or mixed. In the preclinical model, SKES-CT showed that AuNPs remained at the site of cell injection, while INPs expanded within and/or along the lesion border, suggesting dissociation of both components in the first days post-administration. Compared to SKES-CT, SPCCT was able to correctly locate gold, but not completely located iodine. When SKES-CT was used as reference, SPCCT gold quantification appeared very accurate both in vitro and in vivo. Iodine quantification by SPCCT was also quite accurate, albeit less so than for gold.

Conclusion: We here provide the proof-of-concept that SKES-CT is a novel method of choice for performing dual-contrast agent imaging in the context of brain regenerative therapy. SKES-CT may also serve as ground truth for emerging technologies such as multicolour clinical SPCCT.

Keywords: Synchrotron K-edge subtraction CT, spectral photon-counting CT, regenerative medicine, cell tracking, neurology

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APA

Tavakoli, C., Cuccione, E., Dumot, C., Balegamire, J., Si-Mohamed, S.A., Kim, J., Crola-da-Silva, C., Chevalier, Y., Boussel, L., Douek, P., Cormode, D., Elleaume, H., Brun, E., Wiart, M. (2023). High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT. Nanotheranostics, 7(2), 176-186. https://doi.org/10.7150/ntno.79575.

ACS

Tavakoli, C.; Cuccione, E.; Dumot, C.; Balegamire, J.; Si-Mohamed, S.A.; Kim, J.; Crola-da-Silva, C.; Chevalier, Y.; Boussel, L.; Douek, P.; Cormode, D.; Elleaume, H.; Brun, E.; Wiart, M. High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT. Nanotheranostics 2023, 7 (2), 176-186. DOI: 10.7150/ntno.79575.

NLM

CSE

Tavakoli C, Cuccione E, Dumot C, Balegamire J, Si-Mohamed SA, Kim J, Crola-da-Silva C, Chevalier Y, Boussel L, Douek P, Cormode D, Elleaume H, Brun E, Wiart M. 2023. High-resolution synchrotron K-edge subtraction CT allows tracking and quantifying therapeutic cells and their scaffold in a rat model of focal cerebral injury and can serve as a reference for spectral photon counting CT. Nanotheranostics. 7(2):176-186.

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