Nanotheranostics 2021; 5(1):27-35. doi:10.7150/ntno.51391 This issue Cite

Review

Imaging of cell-based therapy using 89Zr-oxine ex vivo cell labeling for positron emission tomography

Yutaka Kurebayashi, Peter L. Choyke, Noriko Sato

Molecular Imaging Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

Citation:
Kurebayashi Y, Choyke PL, Sato N. Imaging of cell-based therapy using 89Zr-oxine ex vivo cell labeling for positron emission tomography. Nanotheranostics 2021; 5(1):27-35. doi:10.7150/ntno.51391. https://www.ntno.org/v05p0027.htm
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Abstract

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With the rapid development of anti-cancer cell-based therapies, such as adoptive T cell therapies using tumor-infiltrating T cells, T cell receptor transduced T cells, and chimeric antigen receptor T cells, there has been a growing interest in imaging technologies to non-invasively track transferred cells in vivo. Cell tracking using ex vivo cell labeling with positron emitting radioisotopes for positron emission tomography (PET) imaging has potential advantages over single-photon emitting radioisotopes. These advantages include intrinsically higher resolution, higher sensitivity, and higher signal-to-background ratios. Here, we review the current status of recently developed Zirconium-89 (89Zr)-oxine ex vivo cell labeling with PET imaging focusing on its applications and future perspectives. Labeling of cells with 89Zr-oxine is completed in a series of relatively simple steps, and its low radioactivity doses required for imaging does not interfere with the proliferation or function of the labeled immune cells. Preclinical studies have revealed that 89Zr-oxine PET allows high-resolution in vivo tracking of labeled cells for 1-2 weeks after cell transfer both in mice and non-human primates. These results provide a strong rationale for the clinical translation of 89Zr-oxine PET-based imaging of cell-based therapy.

Keywords: Zirconium-89, Zirconium-89 oxine, cell tracking, positron emission tomography, cell-based therapy


Citation styles

APA
Kurebayashi, Y., Choyke, P.L., Sato, N. (2021). Imaging of cell-based therapy using 89Zr-oxine ex vivo cell labeling for positron emission tomography. Nanotheranostics, 5(1), 27-35. https://doi.org/10.7150/ntno.51391.

ACS
Kurebayashi, Y.; Choyke, P.L.; Sato, N. Imaging of cell-based therapy using 89Zr-oxine ex vivo cell labeling for positron emission tomography. Nanotheranostics 2021, 5 (1), 27-35. DOI: 10.7150/ntno.51391.

NLM
Kurebayashi Y, Choyke PL, Sato N. Imaging of cell-based therapy using 89Zr-oxine ex vivo cell labeling for positron emission tomography. Nanotheranostics 2021; 5(1):27-35. doi:10.7150/ntno.51391. https://www.ntno.org/v05p0027.htm

CSE
Kurebayashi Y, Choyke PL, Sato N. 2021. Imaging of cell-based therapy using 89Zr-oxine ex vivo cell labeling for positron emission tomography. Nanotheranostics. 5(1):27-35.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
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