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Nanotheranostics 2022; 6(1):50-61. doi:10.7150/ntno.63222 This issue Cite

Review

Review on Optical Imaging Techniques for Multispectral Analysis of Nanomaterials

Haeni Lee^1,*, Jaeheung Kim^1,*, Hyung-Hoi Kim^2,✉, Chang-Seok Kim^1,✉, Jeesu Kim^1,✉

1. Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, Busan 46241, Republic of Korea.
2. Department of Laboratory Medicine and Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Busan 49241, Republic of Korea.
^*These authors contributed equally to this work.

✉ Corresponding authors: Jeesu Kim, Ph.D., Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, 63-2 Busandaehag-Ro, Geumjeong-Gu, Busan 46241, Republic of Korea. Phone: +82-51-510-6128; Fax: +82-51-514-2358; E-mail: jeesukimac.kr. Chang-Seok Kim, Ph.D., Department of Cogno-Mechatronics Engineering, College of Nanoscience & Nanotechnology, Pusan National University, 63-2 Busandaehag-Ro, Geumjeong-Gu, Busan 46241, Republic of Korea. Phone: +82-51-510-6116; Fax: +82-51-514-2358; E-mail: ckimac.kr. Hyung-Hoi Kim, Ph.D., Department of Laboratory Medicine and Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, 179 Gudeok-Ro, Seo-Gu, Busan 49241, Republic of Korea. Phone: +82-51-240-7414; Fax: +82-51-510-8153; E-mail: hhkimac.kr More

Abstract

Biomedical imaging is an essential tool for investigating biological responses in vivo. Among the several imaging techniques, optical imaging systems with multispectral analysis of nanoparticles have been widely investigated due to their ability to distinguish the substances in biological tissues in vivo. This review article focus on multispectral optical imaging techniques that can provide molecular functional information. We summarize the basic principle of the spectral unmixing technique that enables the delineation of optical chromophores. Then, we explore the principle, typical system configuration, and biomedical applications of the representative optical imaging techniques, which are fluorescence imaging, two-photon microscopy, and photoacoustic imaging. The results in the recent studies show the great potential of the multispectral analysis techniques for monitoring responses of biological systems in vivo.

Keywords: Multispectral imaging, Nanoparticles, Spectral unmixing, Contrast-enhanced imaging, Biomedical imaging

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