Nanotheranostics 2018; 2(4):320-346. doi:10.7150/ntno.23826 This issue Cite

Research Paper

Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?

Valeriy M Paramonov1,2,4, Diti Desai3, Helene Kettiger3, Veronika Mamaeva2,4†, Jessica M Rosenholm3, Cecilia Sahlgren2,4,5✉, Adolfo Rivero-Müller1,2,4,6✉

1. Institute of Biomedicine, Research Centre for Integrative Physiology and Pharmacology, University of Turku, Finland
2. Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Finland
3. Pharmaceutical Sciences Laboratory, Faculty of Science and Engineering, Åbo Akademi University, Finland
4. Faculty of Science and Engineering, Cell Biology, Åbo Akademi University, Finland
5. Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, The Netherlands
6. Department of Biochemistry and Molecular Biology, Medical University of Lublin, Poland.
Deceased 06 January 2018

Citation:
Paramonov VM, Desai D, Kettiger H, Mamaeva V, Rosenholm JM, Sahlgren C, Rivero-Müller A. Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?. Nanotheranostics 2018; 2(4):320-346. doi:10.7150/ntno.23826. https://www.ntno.org/v02p0320.htm
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Abstract

Graphic abstract

The concept of delivering nanoformulations to desired tissues by means of targeting membrane receptors of high local abundance by ligands anchored to the nanocarrier has gained a lot of attention over the last decade. Currently, there is no unanimous opinion on whether surface functionalization of nanocarriers by targeting ligands translates into any real benefit in terms of pharmacokinetics or treatment outcomes. Having examined the published nanocarriers designed to engage with somatostatin receptors, we realized that in the majority of cases targetability claims were not supported by solid evidence of targeting ligand-targeted receptor coupling, which is the very crux of a targetability concept. Here, we present an approach to characterize targetability of mesoporous silica-based nanocarriers functionalized with ligands of somatostatin receptors. The targetability proof in our case comes from a functional assay based on a genetically-encoded cAMP probe, which allows for real-time capture of receptor activation in living cells, triggered by targeting ligands on nanoparticles. We elaborate on the development and validation of the assay, highlighting the power of proper functional tests in the characterization pipeline of targeted nanoformulations.

Keywords: targeted nanoparticles/nanopharmaceuticals, targetability, ligand-receptor interaction, somatostatin receptor, cAMP, mesoporous silica.


Citation styles

APA
Paramonov, V.M., Desai, D., Kettiger, H., Mamaeva, V., Rosenholm, J.M., Sahlgren, C., Rivero-Müller, A. (2018). Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?. Nanotheranostics, 2(4), 320-346. https://doi.org/10.7150/ntno.23826.

ACS
Paramonov, V.M.; Desai, D.; Kettiger, H.; Mamaeva, V.; Rosenholm, J.M.; Sahlgren, C.; Rivero-Müller, A. Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?. Nanotheranostics 2018, 2 (4), 320-346. DOI: 10.7150/ntno.23826.

NLM
Paramonov VM, Desai D, Kettiger H, Mamaeva V, Rosenholm JM, Sahlgren C, Rivero-Müller A. Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?. Nanotheranostics 2018; 2(4):320-346. doi:10.7150/ntno.23826. https://www.ntno.org/v02p0320.htm

CSE
Paramonov VM, Desai D, Kettiger H, Mamaeva V, Rosenholm JM, Sahlgren C, Rivero-Müller A. 2018. Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?. Nanotheranostics. 2(4):320-346.

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