Nanotheranostics 2018; 2(3):211-221. doi:10.7150/ntno.25119

Research Paper

Tumor Theranostics of Transition Metal Ions Loaded Polyaminopyrrole Nanoparticles

Shuyao Li1, Shuwei Liu1, Lu Wang2, Min Lin3 Corresponding address, Rui Ge1, Xing Li2, Xue Zhang1, Yi Liu1, Lening Zhang4 Corresponding address, Hongchen Sun1,2 Corresponding address, Hao Zhang1 Corresponding address, Bai Yang1

1. State Key Supramolecular Structure and Materials Laboratory, College of Chemistry, Jilin University, Changchun 130012, P. R. China;
2. The Oral Pathology Department, School and Hospital attached to Stomatology, Jilin University, Changchun 130021, P. R. China;
3. Collaborative Innovation Center attached to Marine Biomass Fibers, Shandong Province Materials and Textiles, Marine Biobased Materials Institute, Materials Science and Engineering School, Qingdao University, Qingdao 266071, P. R. China;
4. Department of Thoracic Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Li S, Liu S, Wang L, Lin M, Ge R, Li X, Zhang X, Liu Y, Zhang L, Sun H, Zhang H, Yang B. Tumor Theranostics of Transition Metal Ions Loaded Polyaminopyrrole Nanoparticles. Nanotheranostics 2018; 2(3):211-221. doi:10.7150/ntno.25119. Available from http://www.ntno.org/v02p0211.htm

Abstract

Polypyrrole (PPy) nanoparticles (NPs) possess high near-infrared absorption and good biosafety, showing the potentials as photothermal therapeutic materials. However, the single function and the weak diagnostic function limit the further combination with other functional units to achieve theranostics. In this work, polyaminopyrrole (PPy-NH2) is demonstrated as the alternative of PPy for preparing NPs. Because of the amino group, metal ions, such as Cu(II) and Fe(III) can be loaded in PPy-NH2 NPs, which extends the applications in multimodal theranostics. Systematical studies reveal that the contribution of Cu(II) in multimodal theranostics is greater than Fe(III). Cu can enhance T1 response signal for magnetic resonance imaging (MRI) and be released controllably in the organism, leading to the effect of chemotherapy. Therefore, Cu(II) and Fe(III) co-loaded PPy-NH2 NPs are defined as CuPPy-NH2 NPs. Experimental results indicate that the optimal size of CuPPy-NH2 NPs is 50.2 nm. The photothermal transduction efficiency is 76.4%. After thermochemotherapy, a complete ablation of human oral epithelial carcinoma tumors is observed. No tumor recurrence is found.

Methods: Cu(II) and Fe(III) co-loaded PPy-NH2 NPs are prepared through oxidation polymerization by mixing Py-NH2, CuCl2, and FeCl3 in water under stirring at room temperature, which are defined as CuPPy-NH2 NPs. The as-prepared CuPPy-NH2 NPs are tested with a variety of cell and animal experiments for tumor theranostics.

Results: CuPPy-NH2 NPs have good light stability, photothermal stability, biosafety and low toxicity. The optimal size of theranostic CuPPy-NH2 NPs is 50.2 nm, which present a photothermal transduction efficiency of 76.4%. The doped Cu(II) ions also show chemotherapeutic behavior. After thermochemotherapy, a complete ablation of human oral epithelial carcinoma tumors is observed. No tumor recurrence is found. Because of the unpaired electron in Cu atomic orbits, CuPPy-NH2 NPs also show T1-weighted magnetic resonance imaging.

Conclusions: This kind of transition metal-doped polymer gives a competitive approach for designing and fabricating multimodal theranostic nanodevices, which shows the potential in tumor treatment.

Keywords: polyaminopyrrole nanoparticles, theranostics, magnetic resonance imaging, thermochemotherapy

Introduction

Tumor theranostics is a vitally significant application for nano-medical science [1-7]. Plenty of nanoplatforms, constructing from noble metal nanoparticles (NPs) [8-11], carbon-based materials [12-14], organic compounds [15, 16], polymers [17, 18] and some other inorganic NPs such as Fe3O4 [19, 20], SiO2 [21-24], copper chalcogenides [25-27], are tested as novel agents for tumor diagnosis and combination therapies. Apart from these existing building blocks, transitional metal ions also exhibit theranostic functions [28-31]. For example, unpaired electrons in atomic orbits endow Mn(II), Co(II), Cu(II), Fe(III), and etc. as contrast agents for magnetic resonance imaging (MRI) [32-34], which is a precise and free of radiation imaging technique for the diagnosis of soft tissue lesion [35-38]. Some of the transitional metal ions also carry certain dosage of toxicity, which can be used as chemotherapeutic drugs for tumor treatment [39, 40]. Meanwhile, many transitional metal elements are essential to maintaining body health. Fe is crucial for the hemoglobins [41], Cu is important for blood stream formation [42], Zn influences hormonal level [43], and Co can be used for nerve repairing [44]. However, they are rarely exploited in tumor theranostics. The main hindrance is that metal ions cannot be directed injected in the form of salt or organic molecules chelated compounds. Metal cations can easily disturb the physiological equilibrium, change the activities of proteins and even induce severe toxicity, raising concerns for biosafety [45, 46].

Proper engineering arrangement is required for metal ions before medical applications. Biosafety polymer enveloping is a facile strategy and it has been used for the surface passion of NPs, increasing structural stability as well as surface passivation and reducing toxicity from releasing [47]. One of the frequently used polymers is polypyrrole (PPy) due to the easy fabrication. PPy is a conductive polymer with good biosafety and it is widely used as covering material of Au-based nanomaterials, SiO2 NPs and some supramolecular structures [8, 22]. Additionally, Liu et al. reported pure PPy NPs as sufficient heat generator under NIR laser irradiation for the effective photothermal ablation of malignant tumors [48]. However, PPy NPs still show limitation in several aspects. First, the function of tumor diagnosis should be included. Second, appropriate method is required to track and identify PPy NPs in vivo. Thirdly, though Zhen and his colleges revealed the biodistribution of SiO2@PPy [49], the accumulation of pure PPy NPs in vital organs and tumor needs detailed study for the full estimation after systemic injection. Hence, properly engineered metal ions in PPy NPs would solve aforementioned aspects perfectly. On the one hand, by importing imaging capability from metal ions, PPy NPs can achieve tumor diagnosis and therapy simultaneously [50]. On the other hand, metal elements labeling PPy can provide the feasibility to monitor the change of PPy concentration in vivo through MRI, such as in blood, brain, muscles, and inner visceral organs [51-53].

The tumor theranostic performance is also affected by the doping quantity of metal ions, while the monomer pyrrole (Py) is of low coordination capability to metal ions, resulting into low loading capability of PPy NPs. Moreover, when chemotherapeutic metal ions is loaded, the weak coordination interaction between metal ions and PPy carriers may break up in normal physical conditions, causing damages to normal tissue [54, 55]. Hence, stronger coordination bonding is required to improve the metal ion loading efficiency and the biosafety. In this work, we replace Py with Py-NH2 to increase the coordinative ability with transitional metal elements. Thus, Cu(II) and Fe(III) are loaded in PPy-NH2 (CuPPy-NH2) NPs during the polymerization. Both Cu(II) and Fe(III) can equip PPy-NH2 NPs with MRI tumor diagnosis [39, 56]. Besides, Cu(II) can also label PPy-NH2 NPs and make the NPs traceable in blood and vital organs [57, 58]. After CuPPy-NH2 NPs reach cancerous area based on the enhance permeability and retention (EPR) effect, Cu(II) can be released from NPs and exhibit chemotherapeutic behavior [9]. The onefold tumor inhibition rate for chemotherapy is 70.6 %. Further combining photothermal therapy, the thermochemotherapy can completely ablate tumors without recurrence. The biosafety of CuPPy-NH2 NPs is also carefully explored and the results indicate that CuPPy-NH2 NPs are safe and of high performance in tumor theranostics.

Methods

Materials

All the reagents were commercially available products and used directly without further purification. Deionized water was used directly in all experiments. Propidium iodide (PI) and fluorescein diacetate (FDA) were purchased from Invitrogen. 1-Aminopyrrole (Py-NH2, >98.0%) was purchased from Tokyo Chemical Industry. Ferric trichloridehexahydrate (FeCl3·6H2O), copper chloride dehydrate (CuCl2·2H2O), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazoliumbromide (MTT), mercaptoethylamine (MA, 99+%), mercaptoglycerol, 3-mercaptopropionic acid (MPA, 99+%) and glutathione (GSH, 99%) were got in Sigma-Aldrich. Ammonium hydroxide (NH3·H2O, 25%) was purchased from Beijing Chemical Works.

Preparation of CuPPy-NH2, PPy-NH2 and Cu-loaded polypyrrole NPs

CuPPy-NH2 NPs were prepared by mixing 0.5 mmol Py-NH2, 1 mmol CuCl2, and 4 mmol FeCl3 in 120 mL water under stirring at room temperature for 24 h. By reducing the dosage of Fe(III) from 4.0 to 3.5, 3 and 2.5 mmol, the diameter of CuPPy-NH2 NPs are adjustable from 50.2 ± 5.0 to 20.3 ± 3.0 nm. Through the similar method, PPy-NH2 and Cu-loaded polypyrrole (CuPPy) NPs were also prepared. The products were collected after high speed centrifugation for 20 min under 2000 rcf (g's). The NPs were dispersed in deionized water for further tests and characterizations.

Metal ion release

5 mg/mL CuPPy-NH2 NPs aqueous solution were mixed with 10 mM ammonia (-NH2), sodium citrate (-COOH) and mercaptoglycerol (-SH), respectively. At different time intervals, through high speed centrifugation the CuPPy-NH2 NPs were discarded. The released dose of ions in supernatant was determined by inductive coupled plasma atomic emission spectrometer (ICP-AES).

Cytotoxicity test and the photothermal effect in vitro

The human oral epithelial carcinoma (KB) cells were incubated with different concentrations of CuPPy-NH2 NPs. After 24 h in standard cell media, the cell viability for KB cells was determined by a standard MTT assay on 96-well plates. The MTT test was measured by the optical density (OD) at 490 nm. As for the in vitro of photothermal therapy, KB cells were incubated with the concentration of 50 μg/mL CuPPy-NH2 NPs for 30 min. Then each sample was irradiated at different power densities by an 808 nm NIR laser for 8 min. As for the control group, cell viability was tested at the same conditions without adding CuPPy-NH2 NPs. Each experiment was repeated for five times.

PI and FDA co-staining assay

The treatment efficacy was revealed by PI and FDA. 30000 KB cells were seeded in a 12-well plate. The KB cells were incubated for 30 min with the concentration of 50 μg/mL CuPPy-NH2 NPs containing 100 μg/mL of GSH in cell culture and incubated with cells. Each plate was irradiated at 0.33 W/cm2 for 0, 3, 8, and 10 min, respectively, by an 808 nm laser. After that, 1 μg/mL PI and FDA were added into the KB cell culture in sequence with incubation for 15 min and 30 s, respectively. Finally, fluorescent photographs were taken under the excitation of 488 and 543 nm, respectively.

Animal experiments

4-6 weeks' old balb/c. nude (weighing ~18g) were purchased from Beijing Huafukang Biological Technology Co. Ltd. The mice were used under protocols approved by Jilin University Laboratory Animal Center. After one week's observation, their weights got a basic normal value around 19 g. 1.5 × 106 of KB cells dispersed in 100 μL of cell culture were injected subcutaneously into the right back leg of the mice. The tumor size was measured by a digital caliper every day. When the average tumor size reached ~100 mm3, the mice were allocated into four groups: control group, laser only group, chemotherapy (CuPPy-NH2 NPs only) group, and thermo-chemotherapy (laser + CuPPy-NH2 NPs) group. Moreover, the mice were administrated with CuPPy-NH2 NPs by intravenous (i.v.) injection. The chemotherapy group and thermo-chemotherapy group were injected with 50 μL 1 mg/mL CuPPy-NH2 NPs. And as for the control group and laser only group were intravenously injected with same volume of saline. The tumors in laser only and thermo-chemotherapy group were irradiated for 20 min by an 808 nm laser at 0.33 W/cm2 two days after injection. In addition, the sizes of the tumor and the weights of the mouse were measured every other day. The tumor volume was calculated by the following formula:

V=1/2·L·D2

(L (mm) = the tumor sizes in long axes; D (mm) = the tumor sizes in short axes). After CuPPy-NH2 NPs injection for 16 days, the tumors of the treatment groups and the control group above were taken for further experiments: surgery, weighted by a scale and taken photos through a camera. The tumors and major organs and tissues (lungs, liver, heart, spleen, and kidneys) from each group were taken after heart perfusion and they were also soaked with formalin for further hematoxylin-eosin (H&E) staining.

Blood circulation and biodistribution

For blood circulation of CuPPy-NH2 NPs, the same dose of blood (8 μL) was collected from each mouse at different time intervals and then dissolved in chloroazotic acid (HCl/HNO3 = 3:1). And the mixed solution was analyzed by ICP-AES to determine the total amount of Cu in the blood. Major organs and tissues (liver, spleen, kidneys and tumor) from balb/c. nude (n=8) were collected at the indicated time point to demonstrate the CuPPy-NH2 NPs biodistribution.

MRI study in vivo

The mice were planted with one tumor in the right side of the back legs. Each mouse was intravenously injected with 50 μL 1 mg/mL of CuPPy-NH2 NPs. 24 h later, the mice were anaesthetized and imaged under 1.5 T magnetic field.

Characterization

The UV-visible (UV-vis) absorption spectrum was recorded using a UV-3600 UV-vis spectrophotometer. Transmission electron microscopy (TEM) was characterized by a Hitachi H-800 electron microscope. The Dynamic light scattering (DLS) measurements were implemented using Zetasizer NanoZS (Malvern Instruments). The tests above were all conducted at room temperature. The infrared thermal images photos were taken by FLUKE infrared Ray (IR) thermal camera. The concentration of Cu was measured by ICP-AES. VG ESCALAB MKII spectrometer with an Mg Kα excitation (1253.6 eV) was used to perform X-ray photoelectron spectroscopy (XPS) investigation.

 Figure 1 

TEM images of the as-prepared CuPPy-NH2 NPs with the diameter of 20.3 ± 3.0 (A), 31.4 ± 8.0 (B), 43.2 ± 4.0 (C), and 50.2 ± 2.0 nm (D). The scale bar in (A)-(D) represents 200 nm. (E) UV-vis absorption spectra of the as-prepared PPy-NH2 and CuPPy-NH2 NPs with different diameter. (F) The light extinction coefficients of CuPPy-NH2 NPs at 808 nm.

Nanotheranostics Image (Click on the image to enlarge.)

Results and Discussion

In our experiment, Cu(II) and Fe(III) chelated PPy-NH2 (CuPPy-NH2) NPs are prepared through oxidative polymerization. By changing the initiating concentration of Fe(III), the obtained NPs are quasi-spherical with the adjustable diameters from 50.2 ± 5.0 to 20.3 ± 3.0 nm (Figure 1A-D). High concentration of Fe(III) is used to initial the oxidative polymerization of Py-NH2, where the molar ratio of Fe(III) to Py-NH2 is 5~8:1. The excessive Fe(III) in the reaction plays three roles, namely, improving the loading quantity of Fe(III) in PPy-NH2 NPs, ensuring the complete polymerization of Py-NH2 and providing good colloidal stability. In aqueous solution, PPy NPs are conventional prepared in polymer stabilizers like poly (vinyl alcohol) considering the easy aggregation for pure PPy NPs [48]. But in our system, Fe(III) gives the essential surface potentials for PPy-NH2 NPs (+ 19 mV) by ionization, which provides the critical electrostatic repulsions among NPs without protonating amino groups. Further increasing the feeding ratio of Fe(III) to Py-NH2 above 9:1, the sizes of NPs would not grow larger than 50 nm because of the fast consumption of monomer (Figure S1). Cu(II) is also loaded during the polymerization under competitive coordination with Fe(III). The loading Cu(II) could hardly change the quasi-spherical morphology and the size of PPy-NH2 NPs (Figure S2A). However, the light absorption is enhanced in large scale from visible to near-infrared (NIR) region after the loading of Cu(II) (Figure 1E). As for the NPs of 50.2, 49.8 nm, light extinction coefficient at 808 nm is 4.90×108 and 3.04×108 M-1cm-1 with and without chelating Cu(II) (Figure 1F). The enhancement of light absorption results from the vis-NIR light extinction ability of cupreous complexes [10, 39]. When amino groups coordinate Cu(II), the complexes exhibit enhanced absorption peak from 0.6 to 0.8 (Figure S3). In addition, the size of NPs also affects the light extinction capability (Figure 1E, F, S4). The light extinction coefficients are 4.22×108, 4.43×108, 4.67×108 and 4.90×108 M-1s-1 for the CuPPy-NH2 NPs of 20.3, 31.4, 43.2 and 50.2 nm, respectively.

The structural information of CuPPy-NH2 NPs is further revealed by XPS. The Fe: Cu ratio of 4:1 is chosen to make the CuPPy-NH2 NPs. As shown in Figure 2, the binding energy of N shows the linkage of N-H, N-Cu and N-Fe at 399.7, 400.7 and 401.2 eV, respectively [59]. In addition, the binding energy of Cu also reveals the linkage of Cu-O and Cu-Cl (Figure 2B) [60], which results from the hydration water and counterions of Cl (Figure 2C) [61]. By comparison, the N spectrum of PPy-NH2 NPs without chelating Cu(II) only shows the linkage of N-H and N-Fe at 399.6, 401.0 eV (Figure S5A) [59]. Moreover, Cu is not covalently bound to PPy NPs. But a slight Cu can still adsorb on PPy NPs, despite the content of Cu is only 0.7%. Due to the low coordinating capability of Py, we could hardly observe the linkage of N-metal from the CuPPy NPs, and the content of Cu(II) cannot be improved in CuPPy NPs (Figure S5A and B). Further combining the ICP-AES data, the formula of CuPPy-NH2 NPs and PPy-NH2 NPs are speculated as [C4N2H4(CuCl2)0.6·(H2O)0.2(FeCl3)0.17]n and [C4N2H4(FeCl3)0.22]n, respectively.

The loading of Cu(II) improves the photothermal performance of PPy-NH2 NPs. As shown in Figure 3, the temperature of aqueous solution containing NPs exhibits dramatic increments while irradiated by an 808 nm laser. When the laser power density is set at 3.5 W/cm2, the increment in temperature is 47.4 ºC for CuPPy-NH2 NPs after 20 min irradiation, while it is only 26.2 ºC for PPy-NH2 without loading Cu(II) (Figure 3A). The temperature increment is also affected by the size of CuPPy-NH2 NPs, since larger NPs possess higher light extinction capability (Figure 1E) [8]. As shown in Figure 3B, the NPs of 50 nm exhibit the best photothermal performance. Within 20 min irradiation, the temperature increment for 50 nm is 3 ºC higher than that of 20 nm (Figure 3B). Besides, the photothermal converting performance is also influenced by the laser power densities and NPs concentrations (Figure 3C and D). When larger sum of NIR light or higher concentration of NPs is applied, the temperature increment is also higher because of collective heating effect [48]. We additionally determined the photothermal transduction efficiency (η) of CuPPy-NH2 NPs, which represents the heat converting efficiency from absorbed light. By recording the real-time temperature of aqueous solution containing CuPPy-NH2 NPs during the heating and cooling procedure, the η of CuPPy-NH2 NPs is calculated as 76.4 %, while it is only 54.0 % for PPy-NH2 NPs without loading Cu(II) (Figure S6). These evidences confirm that Cu(II) is the main contributor to the enhancement of photothermal performance. It should be noted that considering the DLS diameter of 50 nm NPs is 98.0 nm (Figure S7). The real size of NPs may be between TEM observation and DLS measurement in biological experiments because of the complex physiological environment. As reported, the NPs with diameters of 10-100 nm are most suitable for tumor theranostics applications. The CuPPy-NH2 NPs of 50.2 nm is exploited in the following theranostic performance tests.

Since Fe(III) and Cu(II) possess unpaired electrons in the atomic orbits, they can shorten the longitudinal relaxation (T1) of surrounding protons in high-energy of magnetic fields [25, 39]. As revealed in concentration dependent T1-weighted MRI images (Figure 4B), both Cu(II) and Fe(III) could lighten up water under 1.5T magnetic field. And the respective relaxation rate (r1) of Cu(II) and Fe(III) is 0.21 and 0.70 mM-1s-1 as determined by a 500 M nuclear magnetic resonance (NMR) spectrometer (Figure 4A). The loading Fe(III) and Cu(II) would endow the CuPPy-NH2 NPs with enhanced contrasting performance in MRI. CuPPy-NH2 NPs exhibit continuous enhancement of MRI signals with increasing concentrations (Figure 4B). The r1 is tested as 4.72 mM-1s-1 based on the concentration of Cu(II) (Figure 4A), which is higher than clinical used Gd complexes (4.25 mM-1s-1).

The theranostic performance of CuPPy-NH2 NPs is tested with cancerous cells in vitro. KB cells are chosen to evaluate the photothermal ablation efficiency of CuPPy-NH2 NPs. Before incubating CuPPy-NH2 NPs with KB cells, the colloidal stability is first tested towards different physical environment including water, saline, PBS, cell culture with and without serum (Figure 5A). After storage for 7 days, no obvious aggregation is found in all of the solutions except for PBS. However, the aggregated CuPPy-NH2 NPs could be redispersed in PBS for more than 20 h after shaking (Figure S8), which would not hinder the following applications in vitro and in vivo. Besides, CuPPy-NH2 NPs also exhibit good structural stability under laser irradiation. As shown in Figure 5B, the CuPPy-NH2 NPs remain good NIR photothermal converting capability after five cycles of heating up to 80 ºC and cooling down to room temperature. The absorption spectrum also confirms that no obvious change happens after irradiated with higher laser energy (Figure 5C).

 Figure 2 

XPS N 1s (A), Cu 2p3/2 (B), and O 1s (C) spectra of the as-prepared CuPPy-NH2 NPs.

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Then, CuPPy-NH2 NPs and PPy-NH2 NPs are incubated with KB cell at various concentrations for 24 h to evaluate the cytotoxicity. As revealed in Figure 6A, when the feeding concentration is below 100 μg/mL, CuPPy-NH2 NPs could hardly cause any damages to the KB cells and the relative cell viability is high than 95 %. Further increasing the concentration to 200 μg/mL, the cell viability remains 81 %. However, when the concentration reaches 400 μg/mL, only 58 % of the KB cells survived after 24 h, which may result from toxic component of Cu(II). The PPy-NH2 NPs have no obvious damage to KB cells, representing the low cytotoxicity. Hence, in the following photothermal ablation of KB cells, the incubation concentration of CuPPy-NH2 NPs is set at 50 μg/mL to avoid the toxicity caused cell damages. After irradiated by the 808 nm laser for 8 min, the cell viability decreases with growing laser power densities. When the power density increases to 1.8 W/cm2, nearly 50 % of the KB cells are ablated to apoptosis (Figure 6B). Continuously increasing the laser power density to 3 W/cm2, the majority of KB cells are dead and the cell viability decreases to 27 %. By comparison, the KB cells without incubation with CuPPy-NH2 NPs could hardly receive any damages after the same dosage of irradiation. Besides, the apoptotic cells after laser irradiation are further stained by FDA and PI (Figure 6C-F). FDA could only stain living cells with green fluorescence while PI could only stain apoptotic cells into red fluorescence. As exposed under laser irradiation for 0, 2, 4, 8 min, the fluorescent images clearly exhibit that the green fluorescence is gradually replaced by red (Figure 6C-F), indicating the growing number of apoptotic cells. These evidences confirm that CuPPy-NH2 NPs can effectively ablate KB cells under the 808 nm laser irradiation.

 Figure 3 

(A) The temperature increment of PPy-NH2 and CuPPy-NH2 NPs. 1 mg/mL NPs are irradiated by a 3.5 W/cm2 808 nm laser. (B) The temperature increment of CuPPy-NH2 NPs in different sizes. 100 μg/mL NPs are irradiated by a 3.5 W/cm2 808 nm laser. (C) The temperature increment of CuPPy-NH2 NPs under different concentration. 50 nm NPs are tests by 3.5 W/cm2 808 nm laser. (D) The temperature increment of CuPPy-NH2 NPs with different power density of the 808 nm laser. 1 mg/mL 50 nm CuPPy-NH2 NPs are used.

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 Figure 4 

(A) Transverse relaxation time (T1) relaxation rates (r1) for CuPPy-NH2 NPs, Cu(II) and Fe(III). (B) The concentration-dependent T1-weighted MRI under 1.5 T magnetic field for CuPPy-NH2 NPs, Cu(II) and Fe(III).

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After in vitro tests, in vivo experiments are further performed to evaluate the potentials in tumor diagnosis and therapies. CuPPy-NH2 NPs are i.v. injected into mouse bearing KB tumors. 24 h after injection, liver and renal functions are tested to reveal the short term safety of NPs. As shown in Figure S9, the injection of CuPPy-NH2 NPs could hardly disturb the normal functions. Besides, MRI test is applied to trace the distribution of CuPPy-NH2 NPs in tissues and organs (Figure 7). Comparing with blanket controls, the mice injected with NPs exhibit enhanced MRI signals in heart, lungs, kidneys, spleen and liver, as well as other tissues like muscles and lymphs. The NPs are mainly consumed by liver and kidneys, and the accumulation rate is 10.2 and 12.0 injected dose per gram tissue (% ID/g) (Figure 7A). It should be noted that the accumulation of NPs in tumor area is also significant, which favor the diagnosis of tumor. The tumor size, shape and edges are clearly shown in T1-weighted MRI images (Figure 7F). As determined by ICP-AES, the KB tumor uptake rate is 5.7 %ID/g.

In addition to tumor diagnosis in MRI, the CuPPy-NH2 NPs remained in tumor tissue could also perform combination tumor therapies. KB tumor is a fast growing malignant tumor, and the tumors in control group can expand from 100 to more than 2200 mm3 within 16 days without any depressions (Figure 8A and C). When an 808 nm laser is applied to irradiate the tumors without NPs injection at the power density of 0.33 W/cm2, the tumors could hardly observe any damages based on the observation of H&E stained tumor slices, and the average tumor volume could reach more than 2000 mm3 (Figure 8A, D, S10). However, the tumors receive i.v. injection of CuPPy-NH2 NPs exhibit an obvious depression in size, which is attributed from the release of chemotherapeutic Cu(II) (Figure 8A, E). In order to reveal the metal ion release mechanism, we incubate CuPPy-NH2 NPs in various environments containing different functional groups including the -SH, -COOH and -NH2 for 24 h (Figure S11A) [62, 63]. The results show that the released quantity of Cu(II) is the highest in -SH, which may contribute from the strong coordination interactions with -SH [26]. In addition, the release of Cu from the NPs in serum is 3.5% (Figure S12). This indicates that Cu is stable in serum. Since the microenvironment of tumor is acid and rich of GSH, Cu(II) is more likely to release from the carriers after CuPPy-NH2 NPs reaching the tumor area, thus generating chemotherapeutic effects for KB tumors. As observed from the H&E stained tumor slices, the locally damaged tumor tissue with broken cells and vanishing nuclear is the direct factor tumor depressions, and the tumor inhibition rate is 70.6 % comparing with the control group. According to U.S. Food and Drug Administration, the maximum safe laser power density can be applied to animal bodies is 0.33W/cm2. Further combining the laser irradiation to perform thermochemotherapy (TCT), the tumors could be completely ablated under the power density of 0.33 W/cm2 for 20 min (Figure 8A, F). Note that the concentration of CuPPy-NH2 NPs accumulated in the tumor sites is much higher than the injected concentration. So, the power density of 0.33 W/cm2 is enough. The laser irradiation cause dramatic temperature increments in the local tumor area, and within in 16 min, the central part reaches more than 55 ºC. In comparison, for the mice without i.v. injection of NPs, the temperature of tumor area only increases 5 ºC. Since the cancerous cells in tumor tissue could usually bear a mild environment below 43 ºC, large scales of damaged areas are observed from the H&E stained tumor slices after TCT treatments. The destroyed tumor tissue is not recoverable and in the following 2 months, no tumor recurrence is observed from TCT groups. These evidences confirm that the CuPPy-NH2 NPs are of good tumor theranostic performance.

 Figure 5 

(A) Photographs of CuPPy-NH2 NPs solution after incubation in pure water, saline, PBS, cell culture, and cell culture with 10% serum for 7 days. (B) The real-time temperature records of CuPPy-NH2 NPs solution as heating up and cooling down for 5 cycles at the time interval of 15 s. The laser power density is 4 W/cm2, and the concentration of CuPPy-NH2 NPs is 1 mg/mL. The absorption spectra of NPs solution before and after 5 cycles are compared in (C).

Nanotheranostics Image (Click on the image to enlarge.)

We also evaluated the biosafety of CuPPy-NH2 NPs. Since CuPPy-NH2 NPs are positive charged, it is capable to assist the tumor accumulation via EPR effect. The blood circulation half-life is t1/2=1.60 ± 0.3 h by calculating (Figure 7B). This means that the CuPPy-NH2 NPs can be easily captured and removed by mononuclear macrophage system or reticuloendothelium, which avoids the accumulation in the body. After 24 h, the biodistribution of the main organs and tumor are shown in Figure 7c. There is a high value in tumor area. Then, the MRI signal value and the relative MRI signal value of five vital organs and tumor also give the same conclusion revealed in Figure S13. There is a larger response signal in tumor because of the EPR effect. Meanwhile, as shown in Figure S13, there is a high value in the kidney and liver. It proves that NPs mainly exist in the kidney and liver through the circulation of the blood. Besides, the safety of CuPPy-NH2 NPs is further certified by H&E stained. Compared with the control group, the internal organs include heart, liver, spleen, lung and kidneys cannot be fined any changes in the thermo-chemotherapy group (Figure S14). And the weights of the mice of the TCT group are stable (Figure 8B). All of these results indicate that the CuPPy-NH2 NPs are potential safety agents in cancer diagnosis and treatment.

 Figure 6 

(A) The toxicity test of KB cells with CuPPy-NH2 NPs and PPy-NH2 NPs in different concentration. (B) KB cells are incubated with or without 50 μg/mL CuPPy-NH2 NPs for 2 h, and then they are irradiated by an 808 nm laser with the power density of 1.2, 1.8, 2.4 and 3 W/cm2 for 8 min. Fluorescent images of PI and FDA co-staining cells after combined therapy for 0 (C), 3 (D), 8 (E), and 10 min (F), respectively. The scale bar in (C-F) represents 50 μm.

Nanotheranostics Image (Click on the image to enlarge.)
 Figure 7 

(A) Biodistribution of CuPPy-NH2 NPs in KB-tumor-bearing mice at 24 h i.v. by determining the content of Cu(II) with ICP. (B) Blood circulation of CuPPy-NH2 NPs in KB-tumor-bearing mice at 24 h i.v.. (Figure C-F) The MRI after the injection of NPs. (Figure G-J) The MRI before the injection of NPs. Organs identified by (1)-(6) represent heart, liver, spleen, lungs, kidneys and tumor, respectively. The inset color bar from blue to red represents the MRI signal from low to high.

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 Figure 8 

Photothermal therapy of KB tumors in vivo. (A) Relative tumor volume growing trend. (B) Average body weight for each group. (C-F) Photographs of typical mouse bearing tumor model and tumors taken from each group in the 16th day. The scale bar in (C-F) represents 20 mm.

Nanotheranostics Image (Click on the image to enlarge.)

Conclusions

In summary, we demonstrate a convenient and efficient fabrication of CuPPy-NH2 NPs with excellent tumor theranostic performance. The CuPPy-NH2 NPs are prepared through oxidation polymerization at room temperature and their function is enriched by the doping of transition metal ions. Due to the strong absorption in the NIR region, CuPPy-NH2 NPs have the function of photothermal therapy. The doped Cu ions also show the potential of chemotherapy. With unpaired electrons in atomic orbits, Cu ions is able to shorten the T1 of protons and light up the target area in T1-weighted imaging. Furthermore, CuPPy-NH2 NPs have good light stability, photothermal stability, biosafety and low toxicity. This kind of transition metal-doped polymer gives a competitive approach for designing and fabricating multimodal theranostic nanoplatforms.

Abbreviations

PPy: Polypyrrole; NPs: nanoparticles; PPy-NH2: Polyaminopyrrole; MRI: magnetic resonance imaging; CuPPy-NH2: Cu(II) and Fe(III) co-loaded polyaminopyrrole; CuPPy: Cu(II) and Fe(III) co-loaded polypyrrole; EPR: enhance permeability and retention; PI: propidium iodide; FDA: fluorescein diacetate; FeCl3·6H2O: Ferric trichloridehexahydrate; CuCl2·2H2O: copper chloride dihydrate; MTT: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazoliumbromide; MA: mercaptoethylamine; MPA: 3-mercaptopropionic acid; GSH: glutathione; NH3·H2O: Ammonium hydroxide; -NH2: ammonia; -COOH: sodium citrate; -SH: mercaptoglycerol; ICP-AES: Inductively coupled plasma-atomic emission spectrometry; KB: human oral epithelial carcinoma; OD: optical density; i.v.: intravenous; L: the tumor sizes in long axes; D: the tumor sizes in short axes; H&E: hematoxylin-eosin; UV-vis: UV-visible; TEM: transmission electron microscopy; DLS: dynamic light scattering; IR: infrared ray; XPS: X-ray photoelectron spectroscopy; η: the photothermal transduction efficiency; T1: longitudinal relaxation; r1: respective relaxation rate; NMR: nuclear magnetic resonance; TCT: thermochemotherapy.

Acknowledgements

This work was supported by National Natural Science Foundation (51603084), JLU Science and Technology Innovative Research Team 2017TD-06, the Special Project from MOST of China, and the Fundamental Research Funds for the Central Universities. We also thank Animal Experiment Center, College of Life Science, Jilin University for the help in animal experiments.

Supplementary Material

Attachment

Supplementary figures.

Competing Interests

The authors have declared that no competing interest exists.

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Author contact

Corresponding address Corresponding authors: Hao Zhang, PhD, State Key Supramolecular Structure and Materials Laboratory, College of Chemistry, Jilin University, Changchun 130012, P. R. China. Tel: +86 431 85159205; Email: hao_zhangedu.cn. Hongchen Sun, PhD, The Oral Pathology Department, School and Hospital attached to Stomatology, Jilin University, Changchun 130021, P. R. China. Tel: +86 431 88796010; Email: hcsunedu.cn. Min Lin, PhD, Collaborative Innovation Center attached to Marine Biomass Fibers, Shandong Province Materials and Textiles, Marine Biobased Materials Institute, Materials Science and Engineering School, Qingdao University, Qingdao 266071, P. R. China. Tel: +86 431 85159205; Email: linmin900401com. Lening Zhang, PhD, Department of Thoracic Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China. Tel: +86 431 85159205; Email: 951446482com.


Received 2018-1-26
Accepted 2018-4-22
Published 2018-5-15