Full Text | PDF

Share on tweeters Share on facebook Share on linkedin Share on googleplus

Nanotheranostics 2017; 1(3):338-345. doi:10.7150/ntno.20758 This issue Cite

Research Paper

Amplified visual immunosensor integrated with nanozyme for ultrasensitive detection of avian influenza virus

Syed Rahin Ahmed1, Juan C. Corredor2, Éva Nagy2, Suresh Neethirajan1✉

1. BioNano Laboratory, School of Engineering, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
2. Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2W1, Canada

✉ Corresponding author: Prof. Suresh Neethirajan, Email: sneethirca (S. Neethirajan). Tel: 1.519.824.4120 Ext: 53922

Citation:
Ahmed SR, Corredor JC, Nagy É, Neethirajan S. Amplified visual immunosensor integrated with nanozyme for ultrasensitive detection of avian influenza virus. Nanotheranostics 2017; 1(3):338-345. doi:10.7150/ntno.20758. https://www.ntno.org/v01p0338.htm
Other styles

File import instruction

Abstract

Graphic abstract

Nanomaterial-based artificial enzymes or nanozymes exhibit superior properties such as stability, cost effectiveness and ease of preparation in comparison to conventional enzymes. However, the lower catalytic activity of nanozymes limits their sensitivity and thereby practical applications in the bioanalytical field. To overcome this drawback, herein we propose a very simple but highly sensitive, specific and low-cost dual enhanced colorimetric immunoassay for avian influenza A (H5N1) virus. 3,3´,5,5´- Tetramethylbenzidine (TMBZ) was used as a reducing agent to produce gold nanoparticles (Au NPs) with blue colored solution from a viral target-specific antibody-gold ion mixture at first step. The developed blue color from the sensing design was further amplified through catalytic activity of Au NPs in presence of TMBZ-hydrogen peroxide (H2O2) solution in second step. Hence, the developed dual enhanced colorimetric immunosensor enables the detection of avian influenza virus A (H5N1) with a limit of detection (LOD) of 1.11 pg/mL. Our results confirmed that the developed assay has superior sensitivity than the conventional ELISA method, plasmonic-based bioassay and commercial flu diagnostic kits. Proposed sensing method further showed its capability to detect viruses, avian influenza A (H4N6) and A (H9N2) virus, in blood samples with limit of detection of 0.0269 HAU and 0.0331 HAU respectively.

Keywords: Dual color enhancement, Avian influenza virus detection, Gold nanoparticles, Peroxidase mimic

Citation styles

APA
Ahmed, S.R., Corredor, J.C., Nagy, É., Neethirajan, S. (2017). Amplified visual immunosensor integrated with nanozyme for ultrasensitive detection of avian influenza virus. Nanotheranostics, 1(3), 338-345. https://doi.org/10.7150/ntno.20758.

ACS
Ahmed, S.R.; Corredor, J.C.; Nagy, É.; Neethirajan, S. Amplified visual immunosensor integrated with nanozyme for ultrasensitive detection of avian influenza virus. Nanotheranostics 2017, 1 (3), 338-345. DOI: 10.7150/ntno.20758.

NLM
Ahmed SR, Corredor JC, Nagy É, Neethirajan S. Amplified visual immunosensor integrated with nanozyme for ultrasensitive detection of avian influenza virus. Nanotheranostics 2017; 1(3):338-345. doi:10.7150/ntno.20758. https://www.ntno.org/v01p0338.htm

CSE
Ahmed SR, Corredor JC, Nagy É, Neethirajan S. 2017. Amplified visual immunosensor integrated with nanozyme for ultrasensitive detection of avian influenza virus. Nanotheranostics. 1(3):338-345.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image

©2024 Ivyspring International Publisher. Terms of use