Nanotheranostics 2017; 1(2):178-185. doi:10.7150/ntno.18720

Research Paper

Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria

Jinjoo Kim1, 3*, Hansol Kim2, 4*, Ji Ho Park2, 4✉, Sangyong Jon1, 3✉

1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Daejeon 34141, Republic of Korea;
2. Department of Bio & Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Daejeon 34141, Republic of Korea;
3. KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Daejeon 34141, Republic of Korea;
4. KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Daejeon 34141, Republic of Korea.
* These authors contributed equally to this work.

Abstract

The polymerase chain reaction (PCR) has been an essential tool for diagnosis of infectious diseases, but conventional PCR still has some limitations with respect to applications to point-of-care (POC) diagnostic systems that require rapid detection and miniaturization. Here we report a light-based PCR method, termed as photo-PCR, which enables rapid detection of bacteria in a single step. In the photo-PCR system, poly(enthylene glycol)-modified gold nanorods (PEG-GNRs), used as a heat generator, are added into the PCR mixture, which is subsequently periodically irradiated with a 808-nm laser to create thermal cycling. Photo-PCR was able to significantly reduce overall thermal cycling time by integrating bacterial cell lysis and DNA amplification into a single step. Furthermore, when combined with KAPA2G fast polymerase and cooling system, the entire process of bacterial genomic DNA extraction and amplification was further shortened, highlighting the potential of photo-PCR for use in a portable, POC diagnostic system.

Keywords: bacterial detection, diagnosis, gold nanorod, polymerase chain reaction (PCR), photothermal heating.

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How to cite this article:
Kim J, Kim H, Park JH, Jon S. Gold Nanorod-based Photo-PCR System for One-Step, Rapid Detection of Bacteria. Nanotheranostics 2017; 1(2):178-185. doi:10.7150/ntno.18720. Available from http://www.ntno.org/v01p0178.htm